Coming from North:
Highway 14 – Autostrada Adriatica
– Exit at Cesena (150 km from Assisi) and continue to Perugia (E45) until Assisi exit.
Highway Autostrada del Sole A 1
– Exit Valdichiana until you reach Perugia, continue towards Cesena (E45) until Assisi exit.

Coming from the South:
Highway 14 – Autostrada Adriatica
– Exit Civitanova Marche towards Foligno – Perugia until the Assisi exit.
Highway Autostrada del Sole A 1
– Exit Orte, continue on the E45 towards Perugia – Cesena until the Assisi exit.